In our method, only a set of measured data for a photorefractive two-wave mixing set-up with small sinusoidal phase modulation for fixed wavelength is needed.
The monitoring of first and second order transitions in thin polymeric films is based on detectable differentiations of the total energy of a fixed wavelength laser beam incident almost vertically (angle of incidence <5°) onto a thin polymeric film spin coated on a flat reflective substrate.
Laser light of a fixed wavelength shines on the back of one mirror and some leaks through the mirror, entering the "cavity" between the mirrors.
In this technique a rock sample is powdered and irradiated with X-rays of a fixed wavelength.
Both of these investigations had the limitation of having one fixed wavelength, besides the experimental systems employed being complicated and expensive.
For the native MltC crystals, 1996 images were collected at the ESRF synchrotron (Grenoble, France), with an oscillation of 0.1° on beamline ID29, using a Pilatus 6 M detector and fixed wavelength of 1.74709 Å. MltC crystallized in the space group P21, with unit cell parameters of a = 49.87 Å, b = 114.65 Å, c = 61.40 Å, α = γ = 90°, β = 93.46°.
The progress of decolorization of BG dye was monitored using double beam spectrophotometer (Chemito SPECTROSCAN UV 2600) at fixed wavelength of 663 nM.
Then, the corresponding figure of merit is defined as FOM* = max | dI/dn)/I|, which can be used for evaluating the ability of detecting the light intensity change of reflected wave, and I is the intensity of reflected wave at the fixed wavelength.
For a fixed wavelength, the dependence of transmission efficiency of light on the structure parameters is demonstrated in detail.
The general pattern is a relatively fixed wavelength separation of the L and S opsins.
