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This is because the number of fixed sections on moving objects with greater strength will protect users more effectively.
Since this epoch-making study, time-lapse observations of living slices and examinations of fixed sections have become standard techniques used in investigating this problem.
Experimental determination of local trabecular density and osteoclast and osteoblast distributions in the 5T murine model requires histological examination of fixed sections of bone, precluding serial studies of the progression of multiple myeloma in an individual animal.
It was proposed, based on observations of fixed sections of developing mouse brains, that whether the cleavage plane bypasses or bisects the apical membrane was more correlated with proliferative or neurogenic divisions in the apical divisions that were mostly horizontal (Kosodo et al. 2004 and see below).
Tumour perfusion was analysed by visualising fluorescent microspheres in the vasculature of fixed sections using a Zeiss Axiophot HRM Inverted fluorescent microscope (Zeiss Inc., Jena, Germany) and analysed using Image-Pro 6.2 software (Media Cybernetics Inc., Silver Spring, MD, USA).
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Electron microscopic investigations of glutaraldehyde fixed sections showed remaining stratum corneum layers of irregular depth with one partial thin layer remaining with no effect to the stratum granulosum below.
This notion is supported by the observation that 15-lipoxygenase was detected in only some but not all peroxisomes and that its presence correlated with the diffusion of catalase from some but not all peroxisomes in aldehyde fixed sections of rat liver.
We validated the technique on human cardiomyocytes either freshly isolated or selectively excised from fixed sections of human myocardium by Laser Capture Microdissection.
Conclusions: These results are consistent with previously reported data from fixed sections of immunostained samples.
AP activity was detected by incubating 4% PFA fixed sections of embryos/cultured cells with 4.5 μl/ml NBT (BD Bioscience) and 3.5 μl/ml BCIP (BD Bioscience) substrates.
The comparison of the XFI of anaesthetized and fixed sectioned specimens indicated that no large-scale redistribution of the metals had been caused by the sample preparation.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com