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A similar seasonal profile was seen in the fH of fish held in sea-cages.
Pooled mRNA samples from liver of fish held at 13°C were used as drivers in the forward subtractions and as testers in the reverse subtractions.
The microarray analysis indicated that IKKα was significantly up-regulated by pIC at this point only in the spleens of fish held at 10°C (Table 2).
However, it suggests that the differences in response to pIC between fish held at 10 vs. 16°C were not caused by an enhanced sensitivity to double-stranded RNA due to an over-expression of TLR3 in the spleens of fish held at 16°C and injected with pIC.
Like TLR9, DHX58, STAT1, Deltex3, ZNFX1, IL-8 and SACS, PKR did not respond to pIC at 6HPI in fish held at 10°C, but it was significantly up-regulated at the same time point in the spleens of fish held at 16°C and injected with pIC.
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This data set, extending back for about forty years, includes all purse-seine vessels that have captured tuna in the EPO, and characteristics such as length, breadth, gross registered tonnage (GRT), net registered tonnage (NRT), carrying capacity, cubic meters of fish hold capacity, aircraft use, fish-finding electronics, net dimensions, etc.
In the 6 9 months following smoltification, the growth performance of 835 fish held on continuous light (TB) was superior to that of 715 fish returned to short days (TA).
In addition, clustering based on the 339 genes differentially expressed between fish injected with pIC at the different temperatures and sampled at 24HPI, indicates that the mRNA expression profile of pIC-injected fish held at 16°C and sampled 6HPI was most similar to that of fish injected with pIC at 10°C and sampled at 24HPI.
Further, at 24HPI, IL-8 mRNA was 91-fold up-regulated in the spleens of pIC-injected fish held at 10°C, but only ~ 7-fold up-regulated in the spleens of pIC-injected fish held at 16°C, compared with the time- and temperature-matched PBS controls.
In the present work, IL-8 transcript was 11-fold significantly more highly expressed in the spleens of pIC injected fish held at 16°C and sampled at 6HPI compared with 16°C PBS controls.
At 24HPI we detected no significant differences in the mRNA expression of IκBα in fish held at 10°C or 16°C with either the microarray (Table 2) or QPCR studies.
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