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This test demonstrated specificity of the designed primers for the target groups, as single amplicons of expected sizes were found only for the target bacteria.
Moreover, single amplicons of expected sizes were obtained with both primer pairs from various environmental sample types, including aphid cadavers, plant material, and soil.
All the GST-MP deletion mutants were soluble and were of expected sizes.
All the PCR products were of expected sizes and significantly confirmed the differential expression in each comparison.
All genes gave amplicons of expected sizes.
Fragments of expected sizes were obtained and sequenced.
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The primer pair successfully amplified NahAa gene fragment of expected size from plasmid DNA.
The PCR systems produced amplicons of expected size with DNA of most fungi studied, which included members of the Ascomycetes, Basidiomycetes, Zygomycetes and Chytridiomycetes.
All gene specific primers were confirmed to give a single band of expected size.
All amplifications of expected size were cloned using the TOPO® TA cloning kit (Invitrogen).
Products of expected size were sequenced either directly or after cloning into PMD18-T vectors (Takara).
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