The signal was filtered into four narrow subband components of equal bandwidth (band pass filter with 125 Hz frequency band).
It was mentioned in [17] that equal band division of the spectrum yields suboptimal performance and thus it calculated an optimal global band partition.
For a 50% match, two thirds of the bands in two patterns with equal band number must match.
Positive results would then simply be defined by a test band of equal or greater intensity as the single reference band.
Purified AtGAD1 had one prominent and one minor band, MdGAD1 had one major band, and MdGAD2 had two bands of equal prominence (Additional file 2: Figure S2).
The test band was graded as zero if no visual band appeared and graded 1 through 5 for a visualized band of equal intensity as those on the reference card.
As previously reported [ 18], COS BTPC migrated as two distinct bands of equal intensity that transformed into a single faster migrating band when pre-incubated with λ-phosphatase.
The signal intensity of the respective band was estimated with the 'volume rectangular tool' and corrected for background of a band of equal height and size (area) in an empty sample lane.
The signal intensity of the respective band was estimated with the "volume rectangular tool" and corrected versus the background of a band of equal height and size (area) in an empty sample lane.
In light of the intense ~30-33 ~30-33n-specific band and additional ~46-48 kDa non-specific bands of equandor greadditionalsity than HOXA4, we have significant concerns about the use of this antibody in these types of applications.
