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No results of either probe have been announced.
Rac1, Rho or Arf6 depletion did not have major effects on uptake of either probe (Figure 6A).
Ten microliters of either probe hybridisation mix containing blocking DNAs and LSI/WCP Hybridisation Buffer (Abbott Molecular, Inc., Des Plaines, IL) were added to a specimen, and a coverslip was applied with rubber cement sealed around.
After treatment with etoposide (5 μM) for 16 h, or media for healthy cells, the separate cell samples were incubated with 10 μM of either probe 12 or 13, and subjected to epifluorescence microscopy.
No amplification of either probe was seen in the controls, but signal losses were found in 4.5 9.5% and 5.5 10.5% of cells scored per control sample for CEP 8 and LSI p53, respectively.
Membranes were probed with γ-P-ATP end-labelled anti-miR oligonucleotide: anti-parallel miR-146 5′-AACCCATGGAATTCAGTTCTCAGGACAGAG-3′ and PolyT 5′-TTTTTTTTTCTCTGTCC-3′. Hybridization was carried out overnight at 35°C in Church solution containing at least 10 cpm/ml of either probe.
Similar(54)
The latter effect is taken into account by estimating the mean positional intensity decay on each array as a function of either the probe index or the probe's distance to the 3′ end of its target transcript.
CEP17 amplification was defined as the average segmented log2ratio ≥0.5 of either the probes on the p- or q-arm.
Membranes containing the library of synthetic peptides were either probed immediately or stored at −20°C until needed.
We choose these n points as the spatial coordinates of the chiral carbons of either the docked probe peptide or the interacting protein portion determined by HotPoint.
Zebrafish develop approximately 9 12 neuromasts along one side [ 33], which have their counterparts on the opposite side and all of them are stained by either probe.
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