A stability analysis that leads to the design of efficient protocols is presented.
Selection and use of appropriate promoters, selectable markers, and reporter genes has been helpful for development of efficient protocols for transgenic rice in a number of rice cultivars.
Finally, our results might contribute to the development of efficient protocols to generate oligodendrocyte cultures from patient-specific induced pluripotent stem cells [73], which are emerging as a valuable experimental model to study the pathophysiology of different neurodegenerative diseases.
Functional genetic studies are difficult to perform in perennial woody species due to the lack of efficient protocols for mutagenesis, transformation and in vitro regeneration; therefore, understanding the natural variations for traits of interest represents a valuable tool.
The understanding of where, when and how melatonin is produced and acts in distinct brain areas is of clinical relevance since it provides valuable clues to guide the establishment of efficient protocols for the therapeutic use of this hormone in neuroinflammatory diseases.
This is the first comprehensive study of the BRCA1/2 mutation spectrum in Bulgaria and will assist the establishment of efficient protocols for genetic testing and individualized risk assessment for Bulgarian breast/ovarian cancer patients and healthy individuals at a high-risk.
10 To date, numerous studies have focused on the development of efficient protocols to generate hepatocyte-like cells, and several groups, including ours, have already generated hepatocyte-like cells from human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) in vitro.
The establishment of an efficient protocol for vegetative propagation is still a challenge for Cola anomala, a priority target for domestication in West and Central Africa.
Identifying such antibodies, however, is a goal barred by the lack of an efficient protocol for differential screening.
