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Five images were taken of each well.
Five random fields of each well were photographed and counted.
Images from 9 sites from the center of each well were acquired.
The depth of each well is controlled by varying the voltage on tiny electrodes or gates.
A porous polymeric membrane sealed at the bottom of each well accounts for the sensor support.
Determine the OD600 of each well culture after 24 h; they should typically have an OD of 6 7.
The absorbance of each well was measured at a wavelength of 490 nm for 7 continuous days.
The supernatant of each well was collected for cytokine analysis.
The medium of each well was changed twice per week.
The dashed lines show the position of each well.
The drillers will blow hundreds of small holes (called "perforations") in the pipe of each well.
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