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Unwinding rates determined from the slope of each time trace.
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The collection of amplitude samples from each time trace is fitted to a model of two Gaussian modes.
Each recorded time trace of ionic current amplitude from a captured Φ29 DNAP complex is analyzed in the following series of steps: (i) Determine the centers and relative fractions of the two amplitude clusters.
The center frequencies of these spectrums are plotted as a function of time, hence produced the spectrogram of this time trace, shown in Fig. 12.
Our simple procedure was designed in order to maximally detect this characteristic signal without adding the noise found at the end of the time trace.
Also, the range that can be used for the B&H card was small, and there were artifacts at the beginning and the end of the time trace.
For construction of each single-molecule FRET histogram, E values were averaged from the first 300 ms of each of >10 single-molecule time traces for each combination of aptamer variant and buffer condition.
The simulation program also records the completion time of each data block for the generation of processing time traces.
(ii) Extract dwell time samples of each amplitude level from a time trace.
Overposed measurements consist of time traces of the solution or its flux values on a set of discrete points on the boundary ∂Ω.
It is likely that the control group applied a significant portion of their time tracing.
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