Suggestions(1)
Exact(12)
The calcium ionophore ionomycin (1 μM) was added at the end of each time course as an internal assay control.
Samples were collected hourly for the first half of each time course and every other hour thereafter (Additional file 1: Table S1).
Samples were collected for RNA-seq every hour for the first half of each time course, and every two hours thereafter.
At the end of each time course, the potassium ionophore valinomycin was added to a final concentration of 2.5 μM to completely collapse the ∆ψm.
The K+ ionophore valinomycin was added at the end of each time course to cause the complete collapse of the ∆ψm.
Initial velocities were calculated from a linear regression of each time course and corrected for spontaneous ATP hydrolysis and NADH oxidation.
Similar(48)
Again, as with the common genes described above, even within one identified pathway, the profiles of changes in each time course were frequently quite different.
After each time course of study, 100 μl of supernatant was discarded and 20 μl of MTS/PMS solution (Promega, USA) was added, followed by incubation in the dark for 1 hour.
The number of models m depends on the number of time courses tc because each time course introduces an additional perturbation variable.
Then, each time course of body mass was analyzed with a Friedman's analysis of variance.
Each time course of CEA activation exhibits sigmoidal switch-like shape, converging to an elevated steady state.
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