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The purity of each sorted subpopulation was >95% as validated by post-sorting staining with PE-anti Thy1.2 and APC-anti NK1.1.
By removing satellites at the bottom of each sorted list, the search space for satellite acquisition is continuously pruned.
DNA of each sorted single nucleus was amplified by WGA before array CGH was performed.
The genome of each sorted sample was profiled by oligonucleotide comparative genomic hybridization and targeted resequencing of STAG2.
Five separate biological replicates of each sorted cell population were assayed to produce a data set of 20 chips.
DNA of each sorted single nucleus was amplified by whole genome amplification (WGA) using a modified version of the protocol described by Navin [ 36].
Similar(48)
To overcome these obstacles, we have coupled high-dimensional (Hi-D) FACS sorting with unique IgH multiplex PCR technologies, which allow inclusive amplification of IgH transcripts for each sorted B subset and ultimate sequencing of these sequences.
The contamination of sorted fractions by other chromosomes was determined for each sorted run after analyzing 1,000 chromosomes on a microscopic slide.
The frequency of Tregs in each sorted populations was measured by staining for Foxp3 expression in an aliquot of sorted cells.
The spot images of the genes in each sorted data set were searched and organized by RealSpot.
These "overlapping" HSCs therefore share a common gene expression profile for the 43 genes and may represent, at least partially, the subset of true HSCs present in each sorted population.
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