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A linear regression analysis was performed on the resultant CT values to confirm the linearity of each serial dilution and to calculate the PCR amplification efficiency (Table 1 and Supplementary Figure 1).
As such, this provides a profile of each serial entrepreneur's businesses, contribution to the ecosystem (service), insights, and reflections.
DNA from 200 µl of each serial dilution was processed with the QIAamp DNA Blood Mini Kit (QIAGEN, Valencia, CA) as recommended, except that the final centrifugation of the eluate (50 µl) was performed after a 5-min elution.
Five independent repeats of each serial dilution were performed (n = 5) for each DNA extraction method.
Twenty μl of each serial dilution was plated on KB medium + rifampicin (50 μg/mL) plates.
A 1.0 mL aliquot of each serial 10-fold dilution was pipetted and spread on 3M Petrifilm plates.
Similar(42)
The interaction of same/different location with serial order was not significant [ F 3, 21) = 0.5, p >.25], indicating a similar effect of location for each serial position of the target (i.e., the item that was probed).
Equal amount of cells for each serial dilution were spotted on selective medium and pictures were taken after 3 days of growth on the selective medium.
The findings are qualitative in terms of presenting descriptive information for each serial entrepreneur.
During the incubation, the competitors (monomers, dimers with PEG2000 or PEG5000, or dextran conjugates) were serially diluted with PBS-T and 35 μL of each of serial dilution series was mixed with 35 μL of serum of 400 μg/mL total protein in 2× blocking buffer (PBS-T containing 2% BSA).
During incubation, the competitors (monomers, dimers with PEG2000 or PEG5000, or dextran conjugates) were serially diluted with PBS-T and 35 μL of each of serial dilution series was mixed with 35 μL of antibody solution (50 pM anti-FLAG M2 mouse IgG1, 40 nM anti-AQP4 human igG1, or 100 nM anti-ADP3 chicken IgY) in 2× blocking buffer.
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