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MicrOmega/IR illuminates and images samples a few mm in size and acquires the NIR spectrum of each resolved pixel in up to 320 contiguous spectral channels.
Electron cryotomography shows that before septal thinning the cytoplasmic faces of the septum are separated by ∼40 nm (Tocheva et al., 2013), suggesting that the centers of each resolved subcomplexes of SpoIIIE lie in separate cells.
Compounds were identified by comparing the retention times of resolved peaks with those of alkane and fatty acid standards, and by comparing the mass fragment pattern of each resolved peak to the National Institute of Standards and Technology (NIST) 2005 mass spectral library (NIST, Gaithersburg, MD) using the software GC/MS postrun analysis v2.3 (Shimadzu Scientific, Columbia, MD).
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For example, within the M. murinus mtDNA clade the localities of Bemanasy and Mandena were each resolved as monophyletic sets of haplotypes (Fig. 2).
In one study, women who abstained from caffeine during the PMS phase of each month resolved their symptoms completely.
We characterized the likelihood surface of each possible resolved tree using numerical integration over branch lengths.
The methods employed were able to: (1) resolve sources of the individual DOM fluorescence components of the bulk DOM; (2) generate spatial and temporal distribution maps of each of the components on the shelf; and (3) provide insights into biological, chemical, and physical processes that control the DOM variability of each of the resolved DOM components.
Control methods were showed in two aspects (sources and means of transmission), of which each was resolved by corresponding control strategy.
Together with the chemical analysis of the inner part of each deposit, spatially resolved analysis of the interfaces Pt-Co and SiO2-Co has also been performed to understand the differences in chemical composition between the core and the surface.
A total of 20 μg of protein of each sample was resolved in 10% sodium dodecyl sulphate/polyacrylamide gel electrophoresis.
Aliquots of protein (100 μg of protein each) were resolved on a 10% SDS-PAGE gel and transferred to a polyvinylidene difluoride membrane (Millipore, Medford, MA, USA).
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