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The weight and number of seeds were measured for the inner six hills of each replication.
Figure 7 groups the box plots of each replication and clean-up combination including the base case.
The lesion length data generated from the BB resistance screening was averaged across sampling units per entry of each replication.
Twenty wheat plants and their roots were collected from the middle of each replication and shaken gently.
Brown rice grains (specific gravity > 1.06 g/cm3) were collected randomly from all plants from the inner six hills of each replication (Fig. 3b, black hills) for metal concentration analyses.
Pollens were carefully squashed in the dye solution as evenly as possible, and pollen tube was visualized under a microscope at 100× magnification and about 200 pollen grains of each replication were observed.
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More than 100 spores were evaluated from five independent leaves of each genotype for each replication.
All well-matured, green unripe fruits of each of the two plants in each replication of each concentration were harvested over a period of 85 days, with a few fruits in each plant allowed to ripen for later collection.
Successive and cumulative effect of albicidin at each replication cycle eventually resulted in genome erosion.
Every sampling was done at morning 10.00 am and samples of nine randomly selected plants (3 from each replication) of each cultivars were pulled together.
Approximately 200 mg each of rachis and hulls were collected from each replication of NT plants, digested, and the metal concentration was measured.
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