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Tag-SNPs were selected by inspecting the genomic region of each replicated locus for genes within 20 kb or within an LD block of any SNP associated with AMD.
One unique copy of each replicated sample was included in the analysis, preferentially retaining the sample with the higher genotyping call rate.
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Trf of each replicate was obtained by relating normalized transpiration rate with suction.
During any measurement, leaves samples were collected from three random spots of each replicate.
We calculated effort as the approximate number of person hours taken for field sampling and processing of each replicate of each sampling method.
Average coverage values of each replicate were normalized to sequencing effort of SAG B, which had the fewest reads.
Hill curves of each replicate were generated using Origin Data Analysis Software.
Three independent biological replicates were carried out and qPCR of each replicate was performed in triplicate.
We extracted total RNA from 10 female flies of each replicate with three biological replicates of each cross.
For each test, 10 000 permutations were performed and the test statistic of each replicate was calculated.
The mean and the standard deviations for each time of each replicate were calculated.
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