Trf of each replicate was obtained by relating normalized transpiration rate with suction.
During any measurement, leaves samples were collected from three random spots of each replicate.
Yields were obtained from grains harvested in the central lanes of each replicate (8 m2), dried to 13%% of moisture and estimated to 1 ha.
Soil samples collected from three random spots of each replicate were put into aluminum boxes, and dried in an oven at 105 °C for 48 h.
Total RNA was extracted from a 2 g sample of each replicate according to the protocol extraction TRIzol™ Reagent (Invitrogen, Carlsbad, CA, USA) and purified with the RNeasy plant mini kit (Qiagen, Courtaboeuf, France) according to the manufacturer's protocol.
Roots of each replicate were cut into small pieces and then a 1 g sub-sample was rinsed with sterile distilled water, crushed with a sterilized pestle and mortar and added to 9 ml of 0.05% sterile water agar solution.
The following criteria weekly measured for each replicate: 1. Live body weight (LBW): Live body weight of each replicate (contains two pairs of adult pigeon with its squabs) was recorded at the beginning of experiment and then weekly.
(b_{k} sim N 0,{sigma _{b}^{2}})) is the additive random effect of each replicate, and ε k (d)∼N 0,σ 2) is a model error term.
Correlation analysis for triplicate samples by R package revealed that the Pearson correlation coefficient (PCC) among replicates was very high as between 96.1 and 99.6%, which meant the repeatability of each replicate was good.
Average coverage values of each replicate were normalized to sequencing effort of SAG B, which had the fewest reads.
The focal colony of each replicate was located at box A, while the other boxes (B, C, D) remained empty and closed.
