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The area of each outlined region was calculated automatically.
Patch locations were identified by the original researchers; we extracted the centers of patches either directly (when marked on the reconstructions) or by taking the center of mass of each outlined patch.
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The theoretical basis of each is discussed and procedures for application of each are outlined and illustrated with case studies.
Canny edge detection was then used to find the outline of each image of the fly's body, and the centroid of each outline was then used to give the pixel coordinates of each image of the fly.
The shape of each outline was described by a set of 14 Fourier coefficients (seven harmonics per two FC).
The zero harmonic (A0) is proportional to the size of each outline and was used as size estimator thereafter.
Within the center of each outline circle, a horizontally or vertically oriented white line segment (length 1.2˚; 29.3 cd/m) was presented.
An EFA of each outline results in a series of Fourier coefficients, and a PCA of these values encapsulates variation across individuals in a series of orthogonal descriptors of shape.
This first step was completed with a separate summary of each transcript outlining the key points participants made in response to the questions.
The workflow of each of the four outlined processes is depicted in Fig. 1.
Considering each of the outlined areas as a Braille letter, and treating the O's in them as dots, you got the message FEEL THE LOVE.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com