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Library quality and diversity was further assessed by sequencing 20 colonies of each library.
See links to the websites and catalogs of each library.
Once quantified, an equimolar concentration of each library was pooled into a single tube.
Concentration of each library DNA was quantified using KAPA Library Quantification Kits (Roche) according to the manufacturer's instructions.
Equal amounts of each library of 96 were combined to give the final library used for sequencing.
Nucleotide sequences of each library member before and after screening or selection can be obtained through deep sequencing.
Before further analyses, transcriptome data of each library were normalized according to the expression value of two RNA polymerase genes rpoB and rpoC (kuste2957, kuste2958 32.
Quantitative PCR (qPCR) was used to analyze the effective concentration of each library.
The quality of each library was determined using a BioRad Experion (BioRad, Hercules, CA).
In order to ensure the quality of each library, the effective concentration of library must be > 2 nM.
The quantity and the quality of each library were assessed using the Agilent 2100 Bioanalyzer.
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