Exact(5)
The hybridization mix contained 50 ng of each labelled BAC DNA and/or of 20 ng of labelled rDNA probe, 10% dextran sulphate, and 50% formamide in 2xSSC.
Total RNA (200 ng) from individual fish (n = 9 for each control group and n = 6 for each recipient group) were labelled with cyanine 3-CTP, and 1,000 ng of each labelled cRNA were hybridized to microarray slides that were analyzed with an Agilent G2565BA Microarray Scanner according to the manufacturer's protocol.
Five micrograms of each labelled cRNA was hybridized to Agilent 4121A oligonucleotide microarrays (Agilent Technologies) at 60°C overnight.
Briefly 825 ng cDNA of each labelled template was fragmented in the dark and made up to a final volume of 20 μl with nuclease free dH2O.
Before hybridisation, 2 μg of each labelled cRNA product were fragmented and mixed with control targets and hybridisation buffer according to the supplier's protocol (Agilent Technologies).
Similar(55)
The concentration of each labeled peptide was measured before mixing the two different fluorophore-labeled samples using cTCCD as previously described.
We also assume that a set of feature vectors is given by specifying the minimum and maximum numbers of occurrences of each labeled path.
Sections were covered with 100 µl of an hybridization of Helios medium [26] and 3 5×105 dpm of each labeled oligonucleotide.
Dye labeled aRNA aliquots for each hybridization (typically 30 pmol each of Cy3 and Cy5) were vacuum dried together and resuspended in 15 µl hybridization buffer (final concentration of each labeled sample = 2 pmol/µl).
Thirty (30) picomoles of each labeled probe were used for hybridization.
If necessary, surfaces of each labeled object were reduced to 100,000 surfaces.
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