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After fertilization, one zygote of each female gametophyte develops into an embryonic sporophyte.
Genetic analysis of parentage will determine what proportion of females actually lays and the realized reproductive output of each female across clutches in a season.
The behaviour of each female was observed 18 times for 5 min per treatment during the week they were housed in the treatment.
On the 10th day, 20 individuals of each female and male flies randomly collected from corresponding cage and matched in a new cage to allow mating at dark.
The eggs of each female were fertilised by sperm from multiple males.
The base of each ovariole had multiple corpora lutea, indicating the reproductive activity of each female (Fig. 2).
The ovaries of each female were then dissected in phosphate buffered saline (PBS) (pH 7.4) and the epidermis was removed.
Four to 15 embryos were transferred into one oviduct of each female as described by Nagy et al.[30].[30]
The measure consequently represents the contribution of each female to the number of females in the subsequent generation.
A set of sections 4 5 µm thickness of all pieces of each female were stained with hematoxyline and eosin or toluidine blue-borax.
We induced superovulation with exogenous gonadotropins in immature Separase control and mutant mice at 3 weeks of age to evaluate the oocyte production of each female.
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