All samples were serially diluted and 50uL of each dilution was spiral plated onto de Man, Rogosa and Sharp (MRS) agar, (Oxoid, Basingstoke, UK) and M17 agar (Oxoid).
Eight serum samples (four positive and four negative) were diluted 1 9 with PBST, and 100 µl of each dilution was added to the microtitration plate wells.
Organ homogenates were serially diluted ten-fold with PBS and 100 μl of each dilution was plated on Ashdown agar.
Cells were serially diluted in 10-fold steps, and 5 μL of each dilution was spotted on agar plates.
Three replicates of each dilution were tested.
Sowing of each dilution was performed at least three times.
Then, 20 μl of each dilution of nevirapine was added.
Bacterial suspension (50 µL) was mixed with an equal volume of each dilution.
One milliliter of each dilution was spread on petri dishes containing the nutrient medium.
A sample of each dilution, 100 μL, was plated on full media agar for the respective Rhodococci strains.
Serial dilutions were prepared, and 300 μL of each dilution was used for lipid quantification, as described below.
