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Freezing behavior was analyzed for 20 s after the presentation of each conditioned tone.
Fifteen microliters of each conditioned medium and zymography sample buffer were combined and incubated at room temperature for 5 minutes.
Fibroblasts were incubated in 3 ml DMEM containing 2% FCS with 50% of each conditioned medium, 10 ng ml 1 TGF- β (R&D Systems), and 10 μ anti-TGF β neutralising antibody.
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Each symbol represents the mean values of each condition at the end of exercise (N = 8).
The significant interaction effect of condition and hearing lead to the analysis of each condition and each hearing status.
The films were presented in a block design with four repetitions of each condition.
There was a 10-day interval between the examination of each condition.
Participants also completed a web-based survey on the last day of each condition.
All data were the mean values of each condition at the end of exercise (N = 8).
However, the slopes of each condition (i.e. pressure and temperature) are different.
Participants completed three 45-s blocks of each condition, in a counterbalanced order.
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