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Equation (6) is the mathematical basis of multicomponent analysis which permits the determination of the concentration of each compounds without interference from the other components of the mixture.
Standard solutions of each compounds with seven different concentrations were prepared individually in methanol and were injected into LC-MS/MS system for generation of external standard calibration curves.
IC50 values of each compounds was calculated by GraphPad Pro 5.0 (San Diego, CA).
Thus, error values were very less, and the fit value gives a good measure of how the defined features in Hypo1_A and Hypo1_B fit well with the pharmacophore of each compounds.
Therefore, experiment was done to confirm this probability hence, incubation of cancer cells with higher concentrations of each compounds showed higher anticancer activity (EACC was used, data not shown).
Strain MITXM-61 was able to adapt to lignin, 4-HB, and syringaldehyde by incubating on plates in the presence of sublethal concentrations of each compounds as described above.
Similar(53)
In other words, one one-hundredth of the lethal dose of each compound can be fatal when the two are combined.
The purity of each compound exceeded %.
Satisfactory linearity for the analysis of each compound was obtained.
Calculate numerous molecular descriptors of each compound in the datasets.
Twofold serial dilutions of each compound were prepared [26].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com