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Three replicates of each assay were performed.
Advantages and limitations of each assay are discussed.
Three replicates of each assay were performed and germination data were statistically analyzed using SPSS software.
The Youden index was calculated to evaluate the synthetic ability of each assay.
Specific tests are suggested to confirm the specificity of each assay.
Coefficient of variation (CV) of each assay was determined in our laboratory according to the CLSI document EP15-A2 [21].
The normality of TRAb distribution was assessed using the Shapiro Wilk test for the results of each assay.
Animals were approximately 17 g at the end of each assay.
Six miRNA groups were identified between the miRNA expression patterns of each assay.
Relative expression of each assay was calculated as: dCt = Ct geometric)−Ct assay).
150 µl of each assay were injected into the column and eluted with filtered and degassed TBS (pH 7.4).
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