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In another study, inhibiting effects of double strength orange and grapefruit juices on DMBA-induced mammary rat tumorogenesis were compared [75].
A substantial dinner with a liberal quantity of double strength Welsh ale was given to the workmen". Given the size of the barracks, the workmen certainly deserved their "double strength Welsh ale" and if the work really was completed in just one year then it was an amazing feat of engineering and human enterprise.
Samples were also enriched by adding an equal volume of double strength selenite broth, followed by overnight incubation at 37°C.
For this experiment, a tube containing 10 mL of coculture growth medium (equal quantities of double strength of MRS and Mueller-Hinton broths) was inoculated with 10 CFU/mL each of Lactobacillus and Salmonella test isolates [ 22].
An aliquot (10 μl) diluted in equal volume of double strength SDS loading buffer was tested on SDS-PAGE to confirm induction using prestained protein marker as a guide of protein size.
The plates were prepared as follows; 50 μL of four-times strength Iso-Sensitest brOxoidOxoid, UK) was added to the first row (A) of wells and 50 μL of double strength Iso-Sensitest broth was added to all remaining wells.
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American emergency rations in World War I consisted of a quarter ounce (7 grams) packet of double-strength instant coffee, packed one per man in containers with multiple types of foods meant for twenty-four men.
The reactions were carried out for 50 min at 36°C and terminated by the addition of an equal volume of double-strength SDS sample buffer containing β-mercaptoethanol.
Subsequently, the gel was removed and the agarose was overlayed with 20 ml of double-strength nutrient agarose (6% TSB, 1% Bacto-agar, 45°C), and plates were incubated overnight at 37°C.
We mixed 100 mL of carcass rinse with 100 mL of double-strength peptone water (Merck).
The cell pellet was resuspended in ice-cold sterile water for 20 seconds followed by an equal volume of double-strength PBS to restore tonicity.
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