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These changes could be made to a number of different restrictions such as technological restrictions, taxes, or closed areas.
We will never be able to put the world's resources to use with full efficiency so long as business decisions are frustrated by a multiplicity of different restrictions by relatively small nation states that are based on parochial considerations, reflect no common philosophy, and are keyed to no common goal".
We begin, however, with a demonstration of the potential of this method for estimation of the effects of different restrictions frequently used in differential gene expression analyses.
As the number of possible choices increases rapidly for different labels, potentially leading to a huge number of different restrictions, we avoid deciding which copies of a label to remove as follows.
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Shuffling was then repeated with the 6 new modules using a range of different restriction-ligation conditions (experiments ts15, ts16, ts23 to 29, Table 2).
This allows for use of a number of different restriction enzymes that cut genomic DNA with different frequencies, and hence generate a predictable range of flanking genomic DNA that could be isolated.
A number of different restriction-digest methodologies already existed, using high-throughput sequencers.
Based on the occurrence of different restriction sites, it is however theoretically possible to correctly identify these species as well, using only commercially available restriction endonucleases (Table 2).
Subclones representative of different restriction fragment profiles were sequenced, and sequences were compared to the BAC reference sequence to map the position and identity of the SNPs present.
Evidence of different restriction modification systems modulating homologous recombination has been associated with clades of Neisseria meningitidis but evidence of recombination between the clades was still found in this bacterium [ 74].
To verify the absence of undesired genomic rearrangements in the locus and to confirm homologous recombination events, additional Southern blot analysis was performed, digesting the genomic DNA of the positive clones with a number of different restriction enzymes and hybridizing them with different probes (Neo probe and 3′ probe).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com