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However, more detailed further studies are needed to determine whether trivial differences on lineage-specific gene expression observed in EBs of different passages can influence (thereby limits) the differentiation potential of EBs.
The track was in the end built out of three hours of different passages".
This discrepancy is possibly caused by the using of different passages of K562 cells.
Statistical analysis of multiple markers (NCAM, PAX6, IGF2, COL1A1, GATA6, HGF, and Amylase) for three-germ layers showed that linage-specific maker genes displayed relatively constant expression levels among hEBs of different passages, whereas some markers such as SOX1, MSX1, and HGF displayed different expression levels (Fig. 6B).
Human foreskin-derived SKP spheres of different passages or treatments were cytospinned.
It may be caused by the investigation of different passages of cultured cells.
Similar(46)
To address this, we monitored the promoter hypermethylation of the TTG1 gene in Arabidopsis cell suspensions of different passage number.
The value of n for HBE/HELMF studies was determined as the number of experimental repeats on cells of different passage.
Importantly, all experiments were reproduced at least three times using MAM-1 of different passage numbers and overlapping experimental treatments and time points.
Many of our findings mirror those seen in the recent study, despite the use of different passaging conditions, indicating that MA is a reliable method for estimating mutation rates and spectra.
To exclude the possibility that invasiveness was overestimated by distinct proliferation capacity of different passage cells, they were treated with 10 μg/ml Mitomycin C for 2 h at 37°C in a CO2 incubator and subjected to the invasion assay.
More suggestions(15)
of several passages
of different entries
of other passages
of specific passages
of different sections
of different fares
of various passages
of different stages
of different visits
of different instances
of different terminologies
of different clubs
of different actors
of different sensibilities
of different uses
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