Exact(1)
Therefore, a comparison of responses to 5-HT and mCPBG is a simple and effective method of determining expression of 5-HT3B subunins in human 5-HT3 receptors; for example at 30 μM 5-HT and mCPBG current amplitudes at homomeric receptors are equal, but mCPBG-induced responses are fivefold larger in 5-HT3AB receptors.
Similar(59)
The verification was carried out by determining expression of select group of differential expressed transcripts.
(F) TaqMan qPCR was performed to determine expression of mRNA expression of indicated genes.
Spinelli et al. used a candidate gene approach to determine expression of six miRNAs in PBMCs of TB patients and TSTneg individuals [14].
Total RNA was isolated from the brain, spinal cord and thymus of transgenic mice to determine expression of the transgenes.
The ΔΔCt method was used to determine expression of the gene of interest, and the expression of effector cells were set equal to 1 [ 39].
We here sought to determine expression of inhibitory regulators of innate immunity in the circulation and at the primary site of infection in patients with lung TB.
One major measurement of tissue-engineered constructs efficacy and performance is determining expression levels of genes of interest at the molecular level.
When automated, it is capable of determining the expression of a large number of genes.
We have now evaluated LDAs as a means of determining the expression of multiple genes simultaneously in human tissues and cells.
The geNorm software was employed as a means of determining the expression stability of the selected reference genes [ 14].
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