Sentence examples for of cycle threshold from inspiring English sources

After cycling temperatures, the number of cycle threshold for each well was recorded.

All samples were tested in triplicate, and the coefficient of variation (CV) was calculated in the triplicates of cycle threshold (CT) to determine the absence of technical and manipulation errors.

A homologous BRSV cRNA standard was used for quantitation of the mRNA by plotting a standard curve of cycle threshold (Ct) values versus standard 10-fold dilutions of cRNA of known concentrations.

The level of target mRNA was determined by the difference of cycle threshold (Ct) values between the target and loading control.

The amount of the test sample product was interpolated from the standard curve of cycle threshold values generated from known starting copy number of standard MTB DNA.

The values of cycle threshold (Ct) reached a stable level at 11.55, when primers and probe were used at 0.2 µM and 0.1 µM respectively.

The relative amount of each transcript was calculated by a standard curve of cycle thresholds for serial dilutions of cDNA sample and normalized to the amount of actin.

The relative amount for each transcript was calculated by a standard curve of cycle thresholds for serial dilutions of cDNA sample and normalized to the amount of actin.

A standard curve of cycle thresholds using serial dilutions of cDNA samples was established and used to calculate the relative abundance of the target gene in WT and mutant smn samples.

The relative amount for each transcript was calculated by a standard curve of cycle thresholds for serial dilutions of cDNA sample and normalized to the amount of the house-keeping beta-actin levels.

Relative expression values using the average of cycle thresholds of target genes and 18s rRNA were calculated using the 2−ΔΔCt method.