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After this observation he developed an immunization procedure based on the use of crude material from the cowpox lesions.
This was purified by flash column chromatography (SiO2, 1 1 hexanes:DCM) to give 4.5 g of crude material that was recrystallized in hexanes to afford 4′-fluoroisoflavanone (3.4 g, 56%) as a fluffy white solid.
Cooling the solution gave 2 g of crude material as off-white crystals (17%).
MAK-4 contains 7 μg per dried gram of crude material of trans-resveratrol determined by high performance liquid chromatography.
The EtOAc fraction was dried by flash evaporation, and the yield of crude material was up to 5.7 g.
The laboratory studies of the herbal material were based on aqueous extracts of crude material whilst the clinical studies were based on ethanol extracts.
Similar(52)
For decades, weapons buffs have quietly taught themselves ancient hunting techniques by making atlatls, bows, slings and spears out of crude materials.
This is in addition to the requirement for crude material in the form of chemical compounds that we call nutrients.
The total antioxidant activity of MAK-4, measured by a modification of the Ferric Reducing Ability of Plasma (F.R.A.P) assay is 144 μmol per gram of dried crude material.
For example, the actual yield of the pure deuterated product was not reported, and the yield of a crude material was <50%.
Low-temperature calcination of the crude material at 593 K led to the final solid Pt@{walls}SiO2.
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