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For kidney immunohistochemical analysis, ~0.25 cm thick strips of cortex tissue were isolated.
The remaining prismatic volume measured 2.1 x 2.1 x 3.1 mm, a volume which was found representative of cortex tissue [ 19].
Cryo-scanning electron micrographs of cortex tissue showed that both cultivars had similar cell size at the fruitlet stage, but cells were larger in 'Scifresh' than in 'Royal Gala' at the mature stage for fruit of equivalent size.
Cryo-SEM was performed using a Polaron PP2000 Cryo Transfer system (QuorumTechnologies, United Kingdom) attached to a FEI Quanta 250 Scanning Electron Microscope [ 51]. 'Royal Gala' and 'Scifresh' apples were matched for size, and samples of cortex tissue were cut and mounted in sample holders containing a mixture of colloidal graphite and OCT™ compound (Sakursa Finetek, Netherlands).
Because the ripe fruit samples in the fruit development experiment consisted of cortex tissue only we identified only those genes that change by at least 2-fold in cortex (after excluding 25 genes with very low expression), giving a list of 456 genes that respond strongly to ethylene in fruit cortex.
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Oxygen diffusion coefficients for cortex tissue of 'Braeburn', 'Kanzi', 'Jonagold', and 'Conference', obtained from previous work on the same cultivars [ 5] are listed in Table 1.
In Nerium oleander druse and prismatic crystals were observed in the adjacent cells of the cortex tissue of the stem.
Each autopsy sample consisted of 300 mg to 500 mg of prefrontal cortex tissue with similar amounts of grey and white matter in each sample.
(I ) Pooled number of caspase 3-positive cells per 10,000 μm of dorsolateral cortex tissue; n = 3 brains from three independent litters and experiments.
These latter findings reveal: (i) the presumed recruitment of other regions to solve recognition memory problems in the absence of perirhinal cortex tissue; and (ii) that these additional recognition mechanisms require more familiarisation trials than perirhinal-based recognition mechanisms.
The effective water conductivity of pear cortex tissue was calculated based on the microscale simulations.
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