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The absolute quantitative standard curve was drawn by plotting △Ct (△Ct = CtGFP − CtTFRC) against the log of GFP gene copies of corresponding standard samples.
This facilitates a comparison of corresponding standard levels and forms that differ among the three agencies.
Results of corresponding standard pairwise meta-analyses were concordant with those of the network meta-analysis.
The amplification efficiency of primers was calculated from the slope of corresponding standard curve.
ME was determined by dividing peak areas recorded for samples spiked after extraction by peak areas of corresponding standard samples prepared in the mobile phase.
Finally, to establish PE, peak areas obtained for samples fortified before extraction were compared to the peak areas of corresponding standard samples prepared in the mobile phase.
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Soluble fermentation products were identified by comparison with retention times and peak areas of corresponding standards.
Quantitative results were obtained by relating the intensities of the separated lipid bands to calibration curves of corresponding standards.
Soluble fermentation products were identified by comparison with retention times and peak areas of corresponding standards as described previously [ 15, 35, 39, 53].
Compounds were identified with retention times of corresponding standards and by comparing the mass spectra on Palisade Complete 600 K Mass Spectral Library (Palisade Mass Spectrometry, USA) and were quantitated with a standard curve.
Quantification of flavonols QDG, QMG and Q was performed by calibration curves of corresponding standards in the same range as above, and yields were expressed as milligrams per gram of fresh weight red onion.
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