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Erroneous sequences were pre-dominantly of correct length and contained single nucleotide substitutions.
A sample was considered positive if there was a visible amplification product of correct length, with correct negative and positive controls.
Colonies containing insert of correct length were cultured and the corresponding plasmids were purified by Qiagen spin miniprep kit (Qiagen) and sequenced.
For instance, one can obtain the same result, corresponding to the quantity c × L in Eq. (1), by using a lower concentration and a longer sample cell of correct length.
Among erroneous sequences of correct length that were not filtered based on phred score, an average of 79%, 88%, and 88% for 5C.C7, OT-1, and DO11.10 had a single error, with progressively decreasing frequency of sequences with greater numbers of errors.
Curiously, initial attempts to amplify full-length cob and cox1 generated multiple PCR products but those of correct length were revealed, by cloning and sequencing, as further linked gene fragments similar to those described above (but containing the cob or cox1 termini as single fragments).
Similar(53)
Within Ditdah, tones of correct lengths are also played using TCCR0 by counting and the Morse code tree, which is constructed out of C pointers, is traversed.
PCR products showed discrete melting curves and specific bands of correct lengths on agarose gels after 40 cycles of amplification.
In this note a procedure for a back-of-the-envelope estimation of the correct length of a single-shielded TBM is reported in the context of a TBM preliminary design phase.
For verification of the correct length of amplification products, they were analysed on an ethidium bromide stained 2% agarose gel.
Virtually all of the erroneous sequences were of the correct length, and this comprised >99% of the error population acquired either with a q = 0 or 30.
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