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Knowledge of all germination options should help to ensure the safe regeneration of conserved material.
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The cutoff threshold for non-conserved material was 30%.
The synteny of the conserved genetic material was well preserved.
Over the long haul it has been the purebreds that have persisted, based partly on the power of conserved traits, the genetic raw materials that old lineages have passed on for millennia.
Length of conserved blocks, repeated units, and motifs are included in supplementary tables S3 S6, Supplementary Material online.
Briefly, overgo probes (see supplementary material for overgo information) were designed for genes at the ends of conserved blocks (or for one gene for blocks smaller than 3 Mb) using the Overgo Maker program developed by The Genome Institute at Washington University.
One family called TR-GAG2, which exhibited a large number of conserved predicted structures (110 elements) as observed in dot-plot and alignment analysis (supplementary data S6, Supplementary Material online), was analyzed further (fig. 4 A and B).
Materials and methods hint at some sort of justification, i.e. the use of conserved orthologous pairs, but there is no reference to BLASTCLUST nor explaination of what is measured by the program.
As part of preliminary work we had used degenerate primers, designed on the basis of conserved segments of the mupirocin ketosynthase (KS) coding regions, to amplify putative thiomarinol KS sequences as described in Materials and Methods.
To understand the origins and evolutionary histories of conserved miRNAs in Gossypium, we tabulated observations from other land plants, using sequences deposited in miRBase 20 (supplementary table S3, Supplementary Material online).
This problem is alleviated by a staggered arrangement of conserved variables.
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