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The absence of common anchor points makes intersubjective utility comparisons generally implausible; therefore, we did not average behavioral and neuronal data across the individual animals studied.
In order to ensure a minimum number of common anchor points among markers, 116 SSR and 1 SNP markers evenly distributed through the melon genome according to two previous linkage maps [ 15, 16] (Additional File 1) were selected to be genotyped in the eight mapping populations.
These software packages take into account all available information from each individual dataset (e.g., population structure and size) and estimate the marker order and genetic distances of common (anchor or bridge) markers using regression mapping (JoinMap) or multiple 2-point maximum likelihood (Carthagène).
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These gaps are likely due to the lack of sufficient common anchor markers in some maps or slight inconsistencies (distance and/or order) among maps.
One of the 3F peptides, 3ZfFLO11/F1, was successfully selected directly from the library Lib12, which contains variants of 3F peptides to target sites bearing the common anchor sequence of GCGG.
For later comparison purposes these tests were linked by a number of common or anchor items.
The 67 items were subdivided to create three versions of the eMeasure, each version comprising 19 unique items and 9 common (anchor) ones.
Although both the number of SNPs obtained and genotyping efficiency can be improved by using an NGS-based genotyping alternative such as GBS [ 50], we chose the Illumina GoldenGate system in v3.5.1 in order to re-use some of the SNPs and thus have common anchoring points with the previous version v3.5 [ 9].
There were very few common anchoring markers between all genetic maps, which impeded a meta-analysis of QTLs, but anchoring on genome allowed aligning all the maps.
Denote by (3) M A = { M A (1 ), M A (2 ),... M A (K A ) } a set of candidate common pleiotropic anchors of A and B. Analogous to Eq. (2), the causal model M A → A → B implies c o r (M A (i ), B ) = c o r (M A (i ), A ) c o r (A, B ).
These markers are basic for studies of the transcriptome of common bean and can form anchor points for genetic mapping studies in the future.
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