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Plants of Columbia background were grown at 23°C in long days.
The mutants of spl, ems1 are of L er background as described [ 22, 32], while the ams mutant is of Columbia background.
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He was always very proud of his Columbia background.
Absence of BRM increases the levels of FLC expression even in the absence of wild-type FRI (Columbia background).
Take advantage of all people around you". And, he reiterated, he and Israel's Columbia background helped them twofold.
Arabidopsis lines, WT (ecotype Columbia-0), and AS11 (CS3861) EMS-induced mutant, and A7 (SAlpcat1456), lpcand (SAlpcat2480) and lpcat2 (SAIL_357_H01) T-DNA insertion mutant lines (all in a Columbia background) were obtained from the Salk Institute via the Nottingham Arabidopsis Stock Centre Universityy of Nottingham, UK).
Consequently, we also constructed a double mutant using the lue1 allele of katanin p60 and the spr2-2 allele, which are both from the Columbia background.
Finally, in a later stage of the study, we succeeded in isolating an equivalent mutant from the Columbia background [ 32] which had the same properties as those described here for lut2.1.
All of the plants used in this study were Arabidopsis thaliana plants in the Columbia background, except for co-2 and gi-3, which were in the Ler background.
All T-DNA insertions were in the Columbia background and appropriate oligonucleotides were used to confirm the presence of the insertions and their homozygous vs. heterozygous state (Additional file 1: Figure S1 and Additional file 1: Table S1) [ 12].
All genetic stocks used in this paper are in Columbia background.
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