Sentence examples for of cold methanol from inspiring English sources

Addition of cold methanol caused a precipitate to form.

Briefly, 100 μl of liposome sample was mixed with 225 μl of cold methanol and 125 μl of chloroform.

For nucleic acid (DNA and RNA) determination, the plant tissues (0.25 g) were homogenized in 2 mL of cold methanol, and insoluble pellet was washed twice with same volume of methanol.

In order to measure tissue concentrations of T3, 300 μl of the tissue was homogenized in phosphate buffer with 6 ml of cold methanol in ice shaken horizontally for 10 min at 4 °C.

Purified PHA were obtained by repeated precipitations in 10 volumes of cold methanol, and analysed by Fourier transform infra red (FTIR), nuclear magnetic resonance (NMR), gas chromatography mass spectrometry (GC MS) and differential scanning calorimetry (DSC) as previously described in Simon-Colin et al. (2008c).

The fixation method used here for staining endogenous EB1 was 4% formaldehyde instead of cold methanol, so that the total EB1 level was revealed.

Cells were then centrifuged (3000  g, 10 min), resuspended in 2 ml of PBS and 3 ml of ice cold methanol, and then stored at −70°C for a minimum of 10 min in order to inactivate choline kinase.

For pigment extraction, additional centrifugation (4000 × g for 5 min) was applied on the aliquot used for the pigment analyses followed by the re-suspension of pellet in 1 ml of 100% cold methanol, followed by 10 min sonication on ice cold water and centrifugation (4000 × g for 5 min).

The membranes were developed in 45 ml of PBS containing 30 ml of 30% (v/v) hydrogen peroxide and 5ml of the chromogenic substrate 4-chloro-1-naphthol (60 mg stock solution of 4-chloro-1-naphthol in 20 ml of ice cold methanol) after three washes in PBST and a final wash in PBS only.

As a control to demonstrate antibody recognition of subsurface proteins, spirochetes were permeabilized by fixation with 1 ml of 100% cold methanol and incubation at −20°C for 20 min. Non-specific binding was blocked by incubation of slides at 30°C for 90 min in blocking buffer (Difco Leptospira Enrichment EMJH, BD, Sparks, MD).

The polymer was precipitated into large volume of ice cold methanol, filtered and dried at high vacuum until a constant weight was reached.