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Using this approach, five layers of cell sheets were stacked sequentially with thicknesses reaching >200 μm.
Porcine MSCs were cultured to a thin layer of cell sheets via osteogenic induction.
Thermo-responsive cell culture carriers are widely used for non-enzymatic harvest of cell sheets.
Transplantation of cell sheets onto damaged hearts improved heart function in several animal models.
The use of cell sheets for tissue engineering applications has considerable advantages over single cell seeding techniques.
Its activity was further studied in a keratinocyte culture system in which fibrin was exposed upon injury of cell sheets.
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A cartoon model of cell sheet formation is depicted in Fig. 3.
The ability of cell sheet detachment was related to the extent of thermosensitivity.
Various types of cell sheet transplantation have improved cardiac function in animal models and clinical settings.
We have performed clinical applications of cell sheet-based regenerative medicine with human patients in several fields.
As a next generation of cell sheet-based tissue engineering, extracellular matrix-mimicked thermoresponsive cell culture surfaces are required for creating functional cell sheets.
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