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Applying our system to lamin-A overexpressing fibrosarcoma cells, we found a markedly reduced stretch of cell area, indicative of a stiffer cytoskeleton.
After 1 h, unbound P-DNA was removed by rinsing and mouse fibroblast cells were plated on the modified substrates and allowed to spread for various times, after which the number of bound cells and their surface area were quantified (for the effect of RGDS density, the availability of the epitope and the analysis of cell area over time see Supplementary Fig. 4).
Title Quantitation of cell area on glass and fibronectin-coated surfaces by digital image analysis.
Cell spreading was measured by evaluation of cell area (upper section).
The value of cell area on 100 nm nanoporous substrates was highly significant when compared to other nanoporous surfaces.
Truskey, George A. and Proulx, Timothy L., Quantitation of cell area on glass and fibronectin-coated surfaces by digital image analysis, Biotechnology Progress, vol. 6 no.
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In most of cell areas, the HAP can provide stable uplink services to users, which are not susceptible to interference from the terrestrial system.
We used Lewis' law [7] – stating that a linear relationship exists between number of neighbors and areas of cells – to compare the distributions of cell areas from our simulations with the distribution of the SAM (Fig. 4).
Quantitative measurements of cell areas clearly showed some mutant cells exhibited a greater extent of cell spreading, that is, R995A/R997A/L1000A, K716A, H722A and R995A/R997A/K716A.
It is notable that each cell population, while distinct from the other populations in the extent of cell spreading, displays a range of cell areas.
Finally, in Table 1, designs are compared according to number of cells, area, and delay.
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