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A single strain of cat cells underwent a change in its restrictive capacity for MSV(FeX) on prolonged passage.
These data reveal rapid Yap1 activation upon exposure of cat cells to methanol.
However, when media contained methylamine, which is oxidized by peroxisomal amine oxidase, the CLS of cat cells was significantly reduced.
As shown in Figure 5H, CCP is essential for survival of cat cells in Gly/MeOH/AS medium.
At these conditions strongly enhanced ROS levels were observed during the exponential growth phase of cat cells.
Conversely, the CLS of cat cells was enhanced relative to the wild-type control, when cells were grown on methanol, which is oxidized by peroxisomal alcohol oxidase.
Similar(47)
However, we now observed that the CLS of the cat cells grown on Glc/MA is reduced relative to the WT control.
Comparison of isozyme profiles of WT and cat cells grown on Glc/MA or Gly/MeOH/AS, revealed that cat cells grown on Gly/MeOH/AS showed the highest SOD1 activity (Fig. 5D), which was inhibited by H2O2 and KCN (Fig. 5E).
Consistent with this is the observed migration of Yap1 to the nucleus upon transfer of glucose-grown cat cells to these media.
To test whether the induction of these genes can overcome the negative effect of MA on cell survival, we compared the CLS of WT and cat cells grown on Gly/MeOH supplemented with MA as sole nitrogen source.
To test this we analyzed the resistance of WT and cat cells to externally added H2O2 or acrolein.
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