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These results verify the conjugation of both strands of double-stranded DNA to AuNPs in DP-1, DP-2, and DP-3.
Sequencing of both strands was performed for the detected mutation.
All constructs were verified by sequencing of both strands.
Internal sequencing primers were designed as needed for complete sequencing of both strands.
Clones were confirmed by restriction analysis and by DNA sequencing of both strands.
The constructs were confirmed for the point mutations by standard DNA sequencing of both strands.
Cloned genes were confirmed by restriction analysis and by DNA sequencing of both strands.
Pioneering studies relied on microarrays consisting of both strands of the targeted genomic regions [2], [15], [16].
The PCR product was fully sequenced, and each nucleotide of both strands was read at least twice.
Primer sequences for amplification and initial sequencing are: 5' tttgccagggtccagttg, and 5' cttggatatacaaagtggtacgt. Full sequences of both strands were obtained using internal sequencing primers.
DNA sequencing of both strands was performed by GENterprise Genomics (Mainz, Germany), followed by analysis on an ABI Prism 310 automated Genetic Analyzer (Applied Biosystems).
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