The FRAP values of adequate concentrations of both extracts (2.5 g/l) indicated in Table 1.
The maximum non-toxic concentrations of both extracts (C. crassa and C. siphonella) were 4.9 and 0.3 μg/ml, respectively).
A complete picture of total antioxidants capacity of both extracts determined was obtained via analysis by FRAP and Ciocalteu assays.
Therefore, it could be concluded that the molecules of both extracts get adsorbed onto both anodic and cathodic sites of the steel surface.
The release behavior of the phenolic compounds from the films in an aqueous medium was assayed finding significant differences (p < 0.05) between release rates of both extracts.
The standard curve was prepared using different concentration (0.1 to 0.5 mg/mL) of gallic acid.. Based on the measured absorbance of the extracts, total phenolic content of both extracts was calculated and expressed in terms of milligram of gallic acid equivalent (GAE) per gram of dry extract.
Investigation of Table 8 reveals that the sign of (eta S_{text{a}}^{^circ }) is negative in free acid solution, whereas it becomes positive with the addition of both extracts, this suggest that the adsorption of organic inhibitor molecules is accompanied by desorption of water molecules from the steel surface [41].
The anticancer activity of both extracts was compared with that of paclitaxel as positive control and the effect was assessed by MTT assay, mitochondrial membrane potential (MMP) alteration, intracellular reactive oxygen species (ROS) measurement and nuclear morphology with the appearance of apoptosis.
By use of a broad spectrum of unbiased analytical methods such as LC-MS, GC-MS of both extracts and headspace, and statistical data analysis tools, the role of metabolic cross-talk between endogenous and ectopic pathways can be uncovered.
A constant amount of both extracts, corresponding to ∼104 cells per reaction, was amplified, in parallel for viral DNA and mRNA, by the Light Cycler (Roche Applied Sciences, Basel, Switzerland) by using Quantitect SYBRgreen PCR and Quantitect SYBRgreen RT-PCR Kits (Qiagen, Hilden, Germany), respectively.
By immunodiffusion, antigens of both extracts give identical reactions.
