Sentence examples for of base call from inspiring English sources

However, the observed sequences include many errors of base call.

Besides the fast and large-scale detection of RNA editing sites, NGS technology has provided depth of coverage (DOC) per reference nucleotide and qualities of base call.

Therefore, if next-generation sequencing is applied to analysis of a heterogeneous population of various mutant sequences, it is necessary to discriminate between true bases as point mutations and errors of base call in the observed sequences, and to subject the sequences to error-correction processes.

The quality score for each base was used as an indicator of base call uncertainty.

Sequence quality was of greater significance: 25% of contigs were removed from the RA on account of base call ambiguity.

For instance, we did not mention the usage of base call or read mapping quality scores in our computations.

Raw reads were subject to quality-based trimming of base calls with Phred quality <26 and reads were removed if more than three low-quality base calls were present in the 28 nt 5' seed region.

Inputs for each variety consisted of a collection of base calls specific to either the phenolic or vitamin E pathway.

Our methods involve use of three different measures: analysis of base calls; analysis of k-mers; and analysis of distributions of k-mers.

The data was put through our sequencing pipeline consisting of base calling using the Illumina Pipeline, mapping and alignment with BWA [15], PCR duplication removal with SAMTools [16], and GATK [17] for variants calling.

On mapping of the reads of GA, mismatch ratio increased with the base position along the reads, which can be due to decrease of fluorescent signal intensities and quality of base calls.