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These results indicate that AP-2α first of all regulates a panel of genes involved in GN-11 migration and proliferation.
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Of all the regulated genes IFN-γ was most up-regulated.
Of all the regulated integrin genes identified, only integrin αV (ITGAV) and ITGB8 were up-regulated in both cell populations (see Supplemental Material, Figure S4).
Of the 27 post-transcripts, expression was low for 11 (all regulate expression of pro-inflammatory genes) and high for 16 (all regulate expression of anti-inflammatory genes) miRNA sequences.
Of all regulated genes in this network only one is up-regulated (Figure 17).
There are four over-represented functional classes consisting of 64 genes, which account for 26.3% of all regulated genes.
The genes regulated in the sample treated with 17β-estradiol (E2) accounted for 90 (40.9%) of all regulated genes, including many genes known to be regulated by E2 such as PGR (progesterone receptor gene), BCL2, FOS and GREB1.
DAVID analysis of all regulated genes and transcripts found by the two analysis techniques yielded 174 common GO-categories (supplemental data, Table S2; cut-off p-value 0.05) again demonstrating the significant common core of both data sets.
In a first approximation the NCE treatment phenotypes (phenocopies) were determined as the total of all regulated genes (p-value <0.01 and |LR| ≥1) comparing NCE treated and TGF-β stimulated cells to DMSO control treated TGF-β stimulated cells.
A detailed description of all regulated genes is listed in Additional file 1.
This result is consistent with the analysis of all regulated genes.
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