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The performance evaluation shows that CUSHAW2 is consistently among the highest-ranked aligners in terms of alignment quality for both single-end and paired-end alignment, while demonstrating highly competitive speed.
Using the above measures, CUSHAW2 is shown to be among the highest-ranked aligners in terms of alignment quality for both the SE and the PE alignment for a variety of error rates and varying amount of indel errors.
The E value describes the probability of the match resulting from chance (a lower value corresponds to a lower probability), and the bit score evaluates the combination of alignment quality and length (a higher value corresponds to a better alignment).The y axis of each diagram shows the negative log10 of the P value and the x axis shows the respective parameter value.
The degree of alignment quality that may be required by a user depends strongly on the intended use of the alignment, and AR alignments could certainly be used as a very good starting point for subsequent shape overlap optimization, e.g., using ROCS.
Small indels can be difficult to identify using Illumina data, so we sequenced a subset of 67 indels, with a variety of alignment quality scores, using traditional Sanger sequencing.
BLOSUM62 scores indicative of alignment quality were averaged over a sliding window of 3 amino acids (i.e. the score at each position represents the average of it and each residue on either side).
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Therefore, in terms of alignment qualities, it is sensible to compare CAMPways with SubMAP.
To exclude areas of low alignment quality, indels of more than 1 amino acid long and 5 flanking sites straddling each such indel were excluded from consideration.
Results obtained were also shown to be an indicator of the alignment quality in the newer version of PASS2.
The SCI (see below) for scoring of structural alignment quality is based on a combination of thermodynamic and covariation measures.
Hence, this option is not advisable for strictly locally related sequences where we observed a reduction of the alignment quality almost down to a score of zero.
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