Boronic acids, 1, have for quite some time now been designed and used as sources of active site-specific, anionic, tetrahedral transition state analogue complexes, 2, of serine amidohydrolases (Scheme 1).
In addition, the selectivity of ciprofloxacin for Mcm2-7 relatove to Mcm467 supports the proposal that active site-specific inhibitors of the Mcm complex can be identified.
These compounds open new possibilities to prepare an array of more active, site-specific or selective antitumor agents.
EPR spectra for KatGNterm and the results of site-specific substitution of active site histidines suggested that the distal histidine was the sixth ligand.
The method is versatile and allows stoichiometric and active site-specific incorporation of any one of many molecular probes.
Therefore, the relative affinities of inhibitors for SFKs with different regulatory domain interactions is a reflection of the importance of active site features and inhibitor substituents toward specific ATP-binding site interactions.
(21, 24, 34, 39, 40) These results illustrate the potential of NRVS to reveal subtle and specific features of active site structure and dynamics in iron-containing proteins more generally.
The SSITKA results show that this deactivation is due to loss of active sites, where the specific site activity of the remaining active sites is unchanged by the water treatment.
Rate constant of active site.
Concentration of active site, mol/l.
Dependence of activity on dispersion is linear for potassium and cesium, whereas in the case of sodium a dramatic decrease of activity is observed when the concentration of sodium surpasses 0.07 0.08 mol% due to the modification of the number or specific activity of active sites.
