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Subconfluent C2C12 cells were infected for 2 h with 100 plaque-forming units/cell of adenovirus vector encoding either myc-DMPK or green fluorescent protein (GFP) before the addition of a suitable volume of myogenic culture media (DMEM with 5% horse serum and antibiotics).
Biomass (20 50 mg dry biomass) was collected by centrifugation of a suitable volume of culture broth, washed with an equal volume of de-ionised water, and stored at −40 or -80°C in Eppendorf Safe-Lock micro-centrifuge tubes.
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Proper volumes of MST WS2 (10 µg/mL) were diluted with a suitable volume of RLMs matrix to prepare eight concentrations: 5, 10, 15, 20, 50, 100, 150 and 200 ng/mL in a final volume of 1 mL.
Therefore, FBA of 75 mL was a suitable volume for the reaction.
The use of cubosomal dispersion was the possible practical solution to attain the required dose of 5-FU in a suitable volume for subcutaneous administration.
Aliquots of midlog phase culture containing the appropriate number of viable bacteria were centrifuged, washed, and resuspended in a suitable volume of DMEM + 10% HI-FCS.
Volumes of 0, 10, 20, 30, 40, 50, 60, 70, 80, 90, and 100 μL of a tannic acid standard solution (0.1 mg/mL) for calibration curve and a suitable volume of extract were introduced into test tubes.
Total RNA was precipitated with 2-propanol, washed once with 70% ethanol, and re-suspended in a suitable volume of DEPC-treated water.
Pellet and resuspend in a suitable volume of 1M sorbitol plus 20mM HEPES.
We mixed the cardiac tissue extracts (40 μg) thoroughly with a suitable volume of PBS buffer and 4 μL dye.
Unbound antibodies were removed by washing with PBS, and cells were resuspended in a suitable volume of buffer.
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