Sentence examples for of a srap from inspiring English sources

A Solexa paired-end sequence was located at the two ends of a SRAP fragment.

Cells were transfected with empty vector or plasmids containing either the full SRA coding sequence and leading to the production of a SRAP-V5 tagged protein [ 16], or a pSuper.retro-SRA construct expressing a SRA-Interfering RNA (SRA-RNAi) SRA-Interfering RNA sequence [ 15], as previously described [ 15, 16].

This polymorphic locus becomes detectable if the amplification efficiencies of two alleles in a SRAP PCR are different enough.

A SRAP profile produced by a pair of SRAP primers always included multiple fragments with different sizes.

Rather, half of the divisions are symmetric producing two SrAp cells, and half of the divisions produce only differentiated cells that ultimately produce sperm and terminate an SrAp lineage.

Illumina's Solexa sequencing is able to produce millions of paired-end sequences and this feature allows the sequencing of thousands of SRAP PCR reactions in one run.

The results suggested that a high level of sequence similarity of SRAP primers reduces the number of detected unique Solexa paired-end sequences.

A total of 91 SRAP markers were identified in the mapping population (Table 2).

Following the discovery of SRAP utility in Citrus, SRAP markers were further exploited to address systematic hypotheses within Citrus.

Through sequencing of tagged SRAP PCR products of a RI line mapping population and their parents, 1,737 Solexa paired-end sequences were integrated in the B rapa genetic map.

PCR of an miRNA-based SRAP marker was performed in a solution (10 μL) containing 30 ng DNA template, 1 × Buffer, 2.0 mmol L-1 MgCl2, 0.2 mmol L-1 dNTPs, 0.1 μmol L-1 of forward primer, 0.1 μmol L-1 of reverse primer, 0.5 units of Taq DNA polymerase, and ddH2O was added to 10 μL.