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Usually, progeny viruses generated by co-infection of two distinguishable parental strains are first cloned by selection of a single plaque and then characterised by PCR.
In addition, analysis of a single plaque over a period of 15.5 months covering the dynamic cubic and the asymptotic phases of plaque development provided further insight into plaque growth characteristics.
For the first time we were able to chart the entire kinetics of growth of a single plaque, and demonstrate a sigmoidal growth curve comprising both a cubic and an asymptotic phase.
The area of a single plaque was calculated as the average lesion thickness (mm) multiplied by the lesion length (mm); and plaque area was calculated as the sum of the area of each plaque (mm).
This finding was corroborated with the recovery of a single plaque that was visualized in toto using a preclinical PET/CT system to show the heterogeneity of tracer distribution ex vivo.
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In addition, to impose the conditions which fostered drift over selection, we used the reliable method of randomly choosing a single plaque (extreme bottleneck of N = 1) to propagate the evolving virus lineage [ 29, 30, 35].
Six out of the seven regions examined were shown to produce amplicons of mixed lengths, despite the template originating from a single plaque purified population of CP220.
A single plaque of each phage with the desired phenotype was used to provide parental stocks for this study.
identified a gene expression signature of inflamed whole plaques which closely overlapped the expression profile of laser microdissected macrophages from a single plaque [10].
The 16S rRNA tree represented in Figure 4 shows the broad diversity of Selenomonas phylotypes recovered in a single plaque sample from a high disease subject (DA).
Plates were read in two ways: microscopic (Microsc), where CPE was defined as even a single plaque of CPE, and with amido black staining (AmidoB), where CPE was defined as 75%% or more of the monolayer gone (is <25% staining).
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