By immunizing with the chain A of ricin (RCA-A), a toxin subunit, we developed a non-human primate (NHP) immune library screened with whole ricin, and we isolated an scFv with picomolar affinity for ricin.
One male macaque was immunised with the A chain of ricin (RCA-A).
A cynomolgus macaque (Macaca fascicularis) (6 kg) was subcutaneously injected with the non toxic A-chain of ricin (RCA-A) purchased from Sigma, Saint Louis, Missouri.
The aim was to evaluate safety and effects on A-B balance of a new RCA-CVVH protocol using 18 mmol/l Citr solution combined with a phosphate-containing hemofiltration (HF) solution.
The aim was to evaluate the effects on electrolyte and acid-base status of a new RCA-CVVH protocol using an 18 mmol/l citrate solution combined with a phosphate-containing replacement fluid, compared with a previously adopted RCA-CVVH protocol combining a 12 mmol/l citrate solution with a conventional replacement fluid.
Because we wanted to search for scFv that would interact with the whole toxin and not A chain alone, and also because of the conformationnal change of RCA-A when coated onto ELISA plates, the library was panned against whole ricin.
The ricin content of crude samples was estimated by SDS-PAGE followed by Coomassie staining and densitometry comparison of unknown RCA-II samples with a RCA-II protein standard curve (data not shown).
Ms. Shawanda's debut album, "Dawn of a New Day (RCA Nashville), released last year, was appealingly rough edged.
These two networks are unlikely to be connected by demographically relevant dispersal within a generation, however, because of a lack of RCAs along the Washington coast that would protect shallow-water rockfish species (Fig. 5, bottom).
Indeed, we found that the extent of binding of a single lectin, RCA-I, alone, was proportional to the metastatic potential of these cells.
